Seahorse XFe 96 Flux Analyzer
Seahorse Overview
The Seahorse XFe96 Extracellular Flux Analyzer simultaneously measures Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) of live cells in a 96-well plate format in order to interrogate changes in oxidative phosphorylation and glycolysis in cultured cells, isolated mitochondria, islets or small tissue samples. This bioenergetic analysis allows for testing of multiple conditions per assay well and direct, simultaneous comparisons across wells, enabling faster throughput with smaller samples compared with traditional respirometry methods.
Training is required before use of this instrument.
Services Offered
| SERVICES | BIDMC | ACADEMIC | INDUSTRY |
|---|---|---|---|
| Basic Assay | $375 | $562.50 | $750 |
| Glycolytic Rate Assay | Upon Request | Upon Request | Upon Request |
| Glycolysis Stress Assay | Upon Request | Upon Request | Upon Request |
| Mito Stress Assay | Upon Request | Upon Request | Upon Request |
| Mito Fuel Flex Test | Upon Request | Upon Request | Upon Request |
| Cell Energy Phenotype Test | Upon Request | Upon Request | Upon Request |
| Training | $185/h | $277.50/h | $370/h |
| Preparing Assay | $32/h | $48/h | $64/h |
| Data Analysis | $158/h | $237/h | $316/h |
If you are interested in using this instrument, please contact Dr. Xiaowen Liu.
Seahorse XF Glycolysis Stress Test
- Measure: glycolytic capacity, glycolytic reserve, and non-glycolytic acidification.
- Kit provides quality-controlled, pre-measured reagents and a standard method for conducting a Seahorse XF Glycolysis Stress Test
- Seahorse XF Report Generator automatically calculates the key parameters of your samples, simplifying data analysis
- Seahorse XF Stress Test Report Generator
- Let’s talk about Glycolytic Activity
Mito Stress Assay
- Measure: Basal respiration, ATP-linked respiration, H+ (Proton) Leak, Maximal Respiration, Spare Respiratory Capacity, and Non-mitochondrial respiration.
- Kit provides quality-controlled, pre-measured reagents and a standard method for conducting a Seahorse XF Cell Mito Stress Test
- Seahorse XF Report Generator automatically calculates the key parameters of your samples, simplifying data analysis
- Seahorse XF Stress Test Report Generators
- Let’s talk about Mitochondrial Respiration
Seahorse XF Cell Energy Phenotype Test
- A routine test for monitoring the Energy Phenotype and Metabolic Potential of live cells
- A simple, sensitive method for comparing metabolic phenotypes between large groups of samples
- Kit provides quality-controlled, pre-measured reagents and a standard method for conducting a Seahorse XF Cell Energy Phenotype Test
- Seahorse XF Report Generator automatically determines the Energy Phenotype and Metabolic Potential of your samples, simplifying data analysis
- Seahorse XF Cell Energy Phenotype Test Report Generator
- Let’s Talk about Seahorse XF Cell Energy Phenotype Test
Seahorse XF Mito Fuel Flex Test
- A single test for analyzing three fuel oxidation pathways: Glucose, Glutamine, and/or Fatty Acid
- Measure: fuel dependency and fuel pathway flexibility
- Kit provides quality-controlled, pre-measured reagents and a standard method for conducting a Seahorse XF Mito Fuel Flex Test
- Seahorse XF Report Generator automatically determines the key parameters of your samples, simplifying data analysis
- Seahorse XF Mito Fuel Flex Test Report Generator
- Let’s talk about Mitochondrial Fuel Flexibility
Scheduling
- Individual consultation and training may be scheduled by contacting the Core.
- First time users should contact at least 2 weeks before running an experiment.
- The maximum amount of time per run is 3 hours (including cartridge calibration).
* It is the responsibility of the user to ensure that the sensor cartridge be hydrated overnight prior to use. The Core has a non-CO2 incubator which can be used for preparation of the cartridge.
Planning Your XFe96 Assay
Materials Required to Run an XFe96 Assay
Procedures Required the Day Before the XFe96 Assay
Any seahorse assay requires extensive preparation prior to the run. Below are resources provided by Agilent to assist in producing a successful run of your experiment. Before your experiment begins the cell culture plate will have to be seeded properly, and the sensor cartridge will need to be hydrated.
Seeding Cells in XFe96 Cell Culture Microplates
Hydrating the Sensor Cartridge for the XFe96 Analyzer
Procedures Required the Day of the XFe96 Assay
Training will cover the preparation of the assay, washing the plate, loading the cartridge with compounds, and how to prepare a run using the seahorse software. Listed below are additional resources covering those areas.
Preparing Assay Media for Use in XF Assays
Washing Cells in XFe96 Cell Culture Microplates
Loading the XFe96/XF96 Sensor Cartridge with Compounds
Running the XF Assay
In order to obtain the best results, optimization of both cell density and FCCP concentration is essential. Our laboratory will work with you to get these variables correct and below are resources detailing the process.
Cell Density and Oligomycin Optimization with the XF Glycolysis Stress Test