Read modes

Absorbance
Fluorescence (top/bottom read)
Luminescence (top read)
Time-Resolved Fluorescence (top read)
Fluorescence Polarization (top read)
HTRF (top read)
AlphaScreen (top read)

Wavelength ranges

Abs: 230 - 1000 nm
FL Ex: 250 - 830 nm
FL Em: 270 - 850 nm
Lumi: 300 - 850 nm

Wavelength selection

Cartridge filters and monochromator tunable in 1 nm increments

Absorbance photometric accuracy/linearity

< ±0.010 OD ±1.0%, 0 to 2 OD

Absorbance photometric precision/repeatability

< ±0.003 OD ±1.0%, 0 to 2 OD

Sensitivity top read optimized

FL: Mono: 0.5 pM fluorescein 96 well, 1 pM fluorescein 384 well; Cartridge: 0.1 fmol/well in 75 μL fluorescein 384 well, 0.03 fmol/well in 8 μL fluorescein 1536 well
Lumi: Mono: 3 pM ATP-Glow 96 well, 6 pM ATP-Glow 384 well; Cartridge: 20 amol ATP-flash
TRF cartridge: 3 amol/well in 100 μL europium (0.03 pM) 384 well, 1 amol/well in 8 μL europium (0.125 pM) 1535 well
FP cartridge: 3 mP (75 μl) fluorescein 384 well, 6 mP (8μl) fluorescein 1536 well
AlphaScreen cartridge: <100 amol/well 384 well

Light source(s)

High power xenon flash lamp
LEDs in visible range

Detector(s)

Silicon photodiode
Cooled photomultiplier tube

Read types (endpoint, kinetic, etc)

Endpoint
Kinetic
Spectrum scan
Well scan

Read times (kinetic interval for 96-well plate)*

Abs: 29 seconds
FL: 22 seconds
Lumi: 21 seconds
TRF: 21 seconds
FP: 38 seconds
AS: 22 seconds

Plate shaking

Linear
Orbital

Plate type(s)

6-1536 well plates

Temperature control

Ambient + 4°C to 45°C

Dimensions

H 32.5 cm x W 39.2 cm x D 60.55 cm

Validation tools

Absorbance validation plate
Fluorescence validation plate
Luminescence validation plate
Multi-mode validation plate
SoftMax® Pro GxP Software