SpectraMax i3x Multi-Mode Microplate Reader

i3x Specifications
Read modes
Absorbance
Fluorescence (top/bottom read)
Luminescence (top read)
Time-Resolved Fluorescence (top read)
Fluorescence Polarization (top read)
HTRF (top read)
AlphaScreen (top read)
Wavelength ranges
Abs: 230 - 1000 nm
FL Ex: 250 - 830 nm
FL Em: 270 - 850 nm
Lumi: 300 - 850 nm
Wavelength selection
Cartridge filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity
< ±0.010 OD ±1.0%, 0 to 2 OD
Absorbance photometric precision/repeatability
< ±0.003 OD ±1.0%, 0 to 2 OD
Sensitivity top read optimized
FL: Mono: 0.5 pM fluorescein 96 well, 1 pM fluorescein 384 well; Cartridge: 0.1 fmol/well in 75 μL fluorescein 384 well, 0.03 fmol/well in 8 μL fluorescein 1536 well
Lumi: Mono: 3 pM ATP-Glow 96 well, 6 pM ATP-Glow 384 well; Cartridge: 20 amol ATP-flash
TRF cartridge: 3 amol/well in 100 μL europium (0.03 pM) 384 well, 1 amol/well in 8 μL europium (0.125 pM) 1535 well
FP cartridge: 3 mP (75 μl) fluorescein 384 well, 6 mP (8μl) fluorescein 1536 well
AlphaScreen cartridge: <100 amol/well 384 well
Light source(s)
High power xenon flash lamp
LEDs in visible range
Detector(s)
Silicon photodiode
Cooled photomultiplier tube
Read types (endpoint, kinetic, etc)
Endpoint
Kinetic
Spectrum scan
Well scan
Read times (kinetic interval for 96-well plate)*
Abs: 29 seconds
FL: 22 seconds
Lumi: 21 seconds
TRF: 21 seconds
FP: 38 seconds
AS: 22 seconds
Plate shaking
Linear
Orbital
Plate type(s)
6-1536 well plates
Temperature control
Ambient + 4°C to 45°C
Dimensions
H 32.5 cm x W 39.2 cm x D 60.55 cm
Validation tools
Absorbance validation plate
Fluorescence validation plate
Luminescence validation plate
Multi-mode validation plate
SoftMax® Pro GxP Software
Source - https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-i3x-readers#specs
Seahorse Scheduling
Appointments for use of this instrument can be made by emailing xliu4@bidmc.harvard.edu
Training
Training is required before use of this instrument.
Individual consultation and training may be scheduled by contacting the Core.
First time users should contact at least 2 weeks before running an experiment.
Links
i3x Pricing
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SERVICES BIDMC ACADEMIC INDUSTRY Customized biomarker panel Upon request Upon request Upon request i3x single use $35 $52.50 $75